This protocol is used to assay the effect of protein over-expression on fitness of . It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of cell expressing this protein.
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| http://dx.doi.org/10.21769/BioProtoc.2396 | DOI Listing |
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