Antigen-specific killing ability of effector CD8 T cells is critical for protective immunity against infection. Here, we describe cytotoxic T cell assay to examine effector function of antigen-specific CD8 T cells. Mice infected with Listeria monocytogenes () expressing chicken ovalbumin as a model antigen mount ovalbumin-specific CD8 T cell responses. Effector CD8 T cell function is determined by mixed transfer of OVA peptide-pulsed target cells with control target cells into the previously immunized mice. Difference in CFSE expression levels clearly marks two distinct populations: Antigen-pulsed target cells-CFSE vs. unpulsed target cells-CFSE. The frequencies between antigen-pulsed target cells and control target cells are used as readouts of antigen-specific killing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697738 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.1172 | DOI Listing |
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