Molecular Identification of and Eggs in Human Populations from Thailand, Lao PDR, and Myanmar.

Am J Trop Med Hyg

Department of Parasitology, Faculty of Medicine, and Research and Diagnostic Center for Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.

Published: January 2018

is a soil-transmitted helminth infecting human populations globally. Human cases caused by and have also been reported. Molecular identifications of species infecting human populations in Lao PDR and Myanmar are lacking. Here, we explored molecular data obtained from eggs recovered from human fecal samples from these countries and compared these with new and existing data from Thailand. Nuclear ribosomal DNA (18S and ITS2) sequences were amplified from eggs and sequenced. Forty-one samples showed 99-100% similarity in their 18S sequences to published sequences of and one sample showed 99% similarity to a sequence of . Similarly, 41 samples showed 92-100% similarity in their ITS2 sequences to published sequences of and one sample showed 94-97% similarity to sequences of . This study is the first molecular confirmation of human infection with in northeast Thailand and the first molecular confirmation of the species of infecting humans in Lao PDR and Myanmar.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928743PMC
http://dx.doi.org/10.4269/ajtmh.17-0651DOI Listing

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