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Highly Stretchable 3D Microelectrode Array for Noninvasive Functional Evaluation of Cardiac Spheroids and Midbrain Organoids.
Adv Mater
December 2024
School of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
Organoids are 3D biological models that recapitulate the complex structures and functions of human organs. Despite the rapid growth in the generation of organoids, in vitro assay tools are still limited to 2D forms. Thus, a comprehensive and continuous functional evaluation of the electrogenic organoids remains a challenge.
View Article and Find Full Text PDFXenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy.
Nat Commun
December 2024
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Article Synopsis
- - The study addresses the limitations of using undefined basement membrane extracts like Matrigel for cultivating intestinal stem cells (ISCs) by introducing a new xenogeneic-free culture dish called XF-DISC.
- - XF-DISC significantly increases the growth and maintenance of ISCs, achieving a 24-fold cell number increase within 30 days and sustaining viability over 210 days (30 passages).
- - This method allows for successful transplantation of cultured human ISCs into mouse models with intestinal injuries, fostering tissue regeneration, making it a promising approach for effective ISC therapy in human intestinal diseases.
SMYD family in cancer: epigenetic regulation and molecular mechanisms of cancer proliferation, metastasis, and drug resistance.
Exp Mol Med
November 2024
Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Epigenetic modifiers (miRNAs, histone methyltransferases (HMTs)/demethylases, and DNA methyltransferases/demethylases) are associated with cancer proliferation, metastasis, angiogenesis, and drug resistance. Among these modifiers, HMTs are frequently overexpressed in various cancers, and recent studies have increasingly identified these proteins as potential therapeutic targets. In this review, we discuss members of the SET and MYND domain-containing protein (SMYD) family that are topics of extensive research on the histone methylation and nonhistone methylation of cancer-related genes.
View Article and Find Full Text PDFHelicobacter pylori VacA-induced mitochondrial damage in the gastric pit cells of the antrum and therapeutic rescue.
Biomaterials
March 2025
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea; KRIBB School of Bioscience, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea; Department of Biological Science, Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address:
Article Synopsis
- The study focuses on VacA, a toxin from Helicobacter pylori, investigating its harmful effects on specific types of stomach cells, particularly how it damages mitochondria and affects cell function.
- Researchers used human gastric organoids (hAGOs) and tissue samples from infected patients to demonstrate that VacA leads to significant mitochondrial damage and reduced energy production, which weakens the stomach's protective barrier.
- The study identified a potential treatment, MLN8054, that can repair VacA-induced mitochondrial damage and restore the integrity of gastric cells, highlighting hAGOs as an effective model for testing new drugs against VacA-related diseases.
Standardization and quality assessment for human intestinal organoids.
Front Cell Dev Biol
September 2024
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives.
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