Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent study.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687092 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2591 | DOI Listing |
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