Background: () is an ornamental plant, used in the traditional medicine and known to have a variety of constituents as alkaloids, flavonoids, and iridoids. Extracts of this plant were proved to have antimicrobial and anticancer activities.
Objectives: This research was conducted for the evaluation of the biological activities of stem bark and isolation and structural elucidation of various chemical compounds from the biologically active fractions.
Materials And Methods: Methanol extract of stem bark of was successively fractionated with petroleum ether, dichloromethane, ethyl acetate, and -butanol. The fractions were evaluated for their antimicrobial, cytotoxic, and antioxidant activities. Fractions with promising biological activities were subjected to chromatographic techniques for the isolation of compounds, followed by structural elucidation using several spectroscopic techniques.
Results: stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against (7.81 μg/ml) and (3.9 μg/ml). The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction, using concentrations of 1, 2.5, 5, and 10 μl/ml. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect, with an IC of 197.1 μg/ml. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5).
Conclusion: was proved to have significant antimicrobial, cytotoxic, and antioxidant activities; the isolated compounds were flavonoids, iridoids, and secoiridoid, some of which were reported for the first time in genus and/or family .
Summary: stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against and . The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). mp: Melting point, NMR: Nuclear magnetic resonance, s: Singlet, d: Double, t: Triplet, q: Quartet, dd: Double-double, m: Multiplet, br: Broad.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669089 | PMC |
| http://dx.doi.org/10.4103/pm.pm_22_17 | DOI Listing |
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