Background: An increase in prevalence of diabetes mellitus necessitates the need to develop new drugs for its effective management. Plants and their bioactive compounds are found to be an alternative therapeutic approach. , used in this study, is well known for its various biological effects.
Objective: The present study was designed to investigate the antihyperglycemic effect of the ethanolic leaf extract of .
Materials And Methods: Different concentrations (1-1000 mg/mL) of the ethanolic leaf extract of were subjected to alpha-amylase and alpha-glucosidase inhibitory assay with acarbose as control. Cytotoxicity was assessed by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Glucose uptake assay was performed on L6 myotubes using the extract in 1 μg-100 μg/mL, using metformin and insulin as control.
Results: The extract showed potent inhibitory activity on enzymes of glucose metabolism in a dose-dependent manner. The maximum alpha-amylase inhibitory effect was 77.37% ± 3.23% at 1000 μg/mL with an IC value of 41.75 μg/mL and alpha-glucosidase inhibitory effect was 83.05% ± 1.69% at 1000 μg/mL with an IC value of 66.71 μg/mL. The maximum glucose uptake was found to be 66.32% ± 0.29% for the extract at 100 μg/mL and that of metformin (10 μg/mL) was 74.44% ± 1.72% and insulin (10 mM) 85.55% ± 1.14%. The extract was found to be safe as the IC of extract and metformin was found to be ≥1000 μg/mL and ≥1000 μM, respectively, in the cell line tested.
Conclusion: The study concludes that has promising antihyperglycemic activity.
Summary: Caralluma fimbriata extract exhibited effective dose dependent inhibitory activity against alpha-amylase and alpha- glucosidaseEnhanced glucose uptake from L6 myotubes was appreciated in the presence of the extract, comparable to Insulin and metforminCaralluma fimbriata has potent antihyperglycemic properties. GLUT: Glucose transporter; MTT: 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide.
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| http://dx.doi.org/10.4103/pm.pm_59_17 | DOI Listing |
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