Flavonoidal Constituents, Antioxidant, Antimicrobial, and Cytotoxic Activities of Grown in Kingdom of Saudi Arabia.

Background: Decne. herb is one of the common traditional plants with multiple medicinal uses.

Objective: To isolate the major constituents and to investigate the antioxidant, antimicrobial, and cytotoxic activities of this herb.

Materials And Methods: Methanolic extract of herb was fractionated using -hexane, dichloromethane, and -butanol. Butanol fraction was chromatographed using column chromatography and preparative thin layer chromatography to isolate the major constituents. Isolated compounds were elucidated by means of spectroscopic methods, including 1D, 2D NMR (H, C, DEPT, COSY, HSQC, HMBC, NEOSY) and MS analysis. Total phenolic content using Folin-Ciocalteu reagent and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay of the total methanolic extract were evaluated. Cytotoxic potential of both methanolic extract and butanol fraction was tested using a crystal violet viability assay. Antimicrobial activities of both extracts were investigated using diffusion agar technique.

Results: Apigenin 6, 8-di-C-glucopyranoside (vicenin-2), quercetin-3`--methyl-3--glucopyranoside, quercetin-3`--methyl-3--galactopyranoside, quercetin-3---D-glucopyranoside, and quercetin-3---D-galactopyranoside were isolated and elucidated. Total phenolic content was (83.89 mg gallic acid equivalent/g extract). The EC value of scavenging DPPH radical was 152.0 ± 2 mg/mL. Butanol fraction showed the highest cytotoxic activity against cervical and breast carcinoma cells (IC 3.6 and 6.1 mg/mL, respectively). Both methanolic extract and butanol fraction showed wide spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria and some fungi. The highest activity was from methanolic extract against (83.25%) and against (77.03%) as compared to reference antibiotics.

Conclusion: Data obtained from this study demonstrate that possesses significant antioxidant, cytotoxic, and antimicrobial activities which could be ascribed to its flavonoidal content.

Summary: Decne. herb is one of the common traditional plants with multiple medicinal uses in KSAFive flvonidal glycosides were isolated and elucidatedThis study demonstrated that possesses significant antioxidant, cytotoxic, and antimicrobial activities. KSA: Kingdom of Saudi Arabia; TLC: Thin Layer Chromatography; DPPH: 2,2`-diphenyl-1-picrylhydrazyl; EC50: Half maximal effective concentration; IC: Half maximal inhibitory concentration; DMSO: dimethyl sulfoxide; NMR: Nuclear Magnetic Resonance; ESIMS: Electrospray ionization mass spectrometry; MeOH: Methyl alcohol.