The current study was to evaluate the protective effects of Chinese medicine prescription Zuogui Pill (ZGP) on osteoporosis (OP) in zebrafish larvae induced by dexamethasone. Alizarin Red staining, calcium and phosphorus determination were performed to evaluate the effect of ZGP on bone mineralization. Hydroxyproline (HP), Alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits. We found that ZGP had positive effects in increasing bone mineral content (BMC), strengthening bone biomechanical, promoting bone formation, inhibiting bone resorption, and mediating protein levels of TGF-β1/Smads signaling pathway. The findings demonstrated that ZGP treatments inhibited the phosphorylation of TGF-β and p-Smad 3 as well as the expressions of collagen I and collagen II by western blot. Taken together, we demonstrated that ZGP may prevent osteoporosis via reversing the imbalance of bone fomation/bone resorption and activating the TGF-β-Smad signal.
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| http://dx.doi.org/10.1016/j.biopha.2017.11.029 | DOI Listing |
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Article Synopsis
- Zuogui Pill (ZGP) is an old Chinese herbal medicine used to help with ovarian aging in women.
- The study looked at how ZGP affects stress in cells and the health of special stem cells in the ovaries of rats that were made old using a drug called cyclophosphamide (CTX).
- Results showed that ZGP made the rats healthier and helped them gain weight and improve their ovary size compared to those affected by CTX.
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