AI Article Synopsis
- The study investigated how elevated temperatures affect tissue banking, specifically focusing on soft tissues like porcine pulmonary heart valve leaflets.
- The research compared leaflets exposed to temperatures of 37 °C, 52 °C, and 67 °C over varying time periods, using multiple assays to measure cell viability, collagen content, and permeability.
- Results showed that higher temperatures significantly decreased cell viability and permeability, while collagen content was less affected, indicating that cell viability assessments are crucial for evaluating living tissues.
Article Abstract
The purpose of this study was to determine the impact of elevated temperature exposure in tissue banking on soft tissues. A secondary objective was to determine the relative ability of various assays to detect changes in soft tissues due to temperature deviations. Porcine pulmonary heart valve leaflets exposed to 37 °C were compared with those incubated at 52 and 67 °C for 10, 30 and 100 min. The analytical methods consisted of (1) viability assessment using the resazurin assay, (2) collagen content using the Sircol assay, and (3) permeability assessment using an electrical conductivity assay. Additionally, histology and two photon microscopy were used to reveal mechanisms of cell and tissue damage. Viability, collagen content, and permeability all decreased following heat treatment. In terms of statistical significance with respect to treatment temperature, cell viability was most affected (p < 0.0001), followed by permeability (p < 0.0001), and then collagen content (p = 0.13). After heat treatment, histology indicated increased apoptosis and two photon microscopy revealed a decrease in collagen fiber organization and an increase in elastin density. These results suggest that measures of cell viability would be best for assessing tissues where the cells are alive and that permeability may be best where cell viability is not intentionally maintained.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797199 | PMC |
| http://dx.doi.org/10.1007/s13239-017-0334-x | DOI Listing |
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Article Synopsis
- Heat stress (HS) prompts neuroinflammation and cognitive issues in the brain, leading to a study that compared the gene expression of mouse hippocampal tissue under HS conditions versus control.
- Analysis showed that HS exposure resulted in 210 differentially expressed genes, indicating significant changes, with 72 genes upregulated and 138 downregulated.
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