We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c7cc05887j | DOI Listing |
Publication Analysis
Top Keywords
luminescence assay
8
kinase activity
8
europium complex
8
atp adp
8
continuous luminescence
4
assay monitoring
4
monitoring kinase
4
activity signalling
4
signalling adp/atp
4
adp/atp ratio
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!