Portable Microplate Analyzer with a Thermostatic Chamber Based on a Smartphone for On-site Rapid Detection.

Anal Sci

Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University.

Published: January 2018

A microplate method provides an efficient way to use modern detection technology. However, there are some difficulties concerning on-site detection, such as being non-portable and time-consuming. In this work, a novel portable microplate analyzer with a thermostatic chamber based on a smartphone was designed for rapid on-site detection. An analyzer with a wide-angle lens and an optical filter provides a proper environment for the microplate. A smartphone app-iPlate Monitor was used for RGB analyze of image. After a consistency experiment with a microtiter plate reader (MTPR), the normalized calibration curves were y = 0.7276x + 0.0243 (R = 0.9906) and y = 0.3207x + 0.0094 (R = 0.9917) with a BCA protein kit as well as y = 0.182x + 0.0134 (R = 0.994) and y = 0.0674x + 0.0003 (R = 0.9988) with a glucose kit. The times for obtaining the detection requirement were 15 and 10 min for the BCA protein kit and the glucose kit at 37°C; in contrast, it required more than 30 and 20 min at ambient temperature. Meanwhile, it also showed good repeatability for detections.

Download full-text PDF

Source
http://dx.doi.org/10.2116/analsci.33.1291DOI Listing

Publication Analysis

Top Keywords

portable microplate
8
microplate analyzer
8
analyzer thermostatic
8
thermostatic chamber
8
chamber based
8
based smartphone
8
on-site detection
8
bca protein
8
protein kit
8
glucose kit
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!