Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063265 | PMC |
| http://dx.doi.org/10.1093/bfgp/elx035 | DOI Listing |
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