AI Article Synopsis
- * Researchers have developed accelerated workflows that link data collection and 3D reconstruction, allowing for efficient cryo-EM applications, such as sample screening.
- * The study demonstrates that the 3D structure of the 70S chlororibosome can be reconstructed to high resolutions (3.2 Å within 24 hours and 3.7 Å in just 80 minutes) using optimized data processing tools, making it feasible for assessing data quality from small samples and potentially applicable in large-scale studies.
Article Abstract
The introduction of direct detectors and the automation of data collection in cryo-EM have led to a surge in data, creating new opportunities for advancing computational processing. In particular, on-the-fly workflows that connect data collection with three-dimensional reconstruction would be valuable for more efficient use of cryo-EM and its application as a sample-screening tool. Here, accelerated on-the-fly analysis is reported with optimized organization of the data-processing tools, image acquisition and particle alignment that make it possible to reconstruct the three-dimensional density of the 70S chlororibosome to 3.2 Å resolution within 24 h of tissue harvesting. It is also shown that it is possible to achieve even faster processing at comparable quality by imposing some limits to data use, as illustrated by a 3.7 Å resolution map that was obtained in only 80 min on a desktop computer. These on-the-fly methods can be employed as an assessment of data quality from small samples and extended to high-throughput approaches.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668856 | PMC |
| http://dx.doi.org/10.1107/S205225251701226X | DOI Listing |
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