Synthetic Biology to Engineer Bacteriophage Genomes.

Methods Mol Biol

LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057, Braga, Portugal.

Published: July 2018

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials.In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.

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http://dx.doi.org/10.1007/978-1-4939-7395-8_21DOI Listing

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