Antibody phage display is the most commonly used in vitro selection technology for the generation of human recombinant antibodies and has yielded thousands of useful antibodies for research, diagnostics, and therapy. The prerequisite for successful generation of antibodies using phage display is the construction of high-quality antibody gene libraries. Here, we give the detailed methods for the construction of human immune and naive scFv gene libraries.
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Exploring Individual Differences in Interoception Among Athletes Based on a Three-Dimensional Construct of Interoception.
Psychophysiology
January 2025
School of Psychology, Beijing Sport University, Beijing, China.
Previous studies demonstrated that sensorimotor training enhances interoceptive abilities. Athletes are highly engaged in performance-driven physical training and often incorporate-to varying degrees-sensorimotor training into their routines. In this study, we investigated the role of individual differences in interoception by comparing professional athletes of different performance levels and both sexes with recreational athletes and controls, applying a three-dimensional model of interoception.
View Article and Find Full Text PDFMid- to long-term clinical outcomes of modified technique skip-level titanium plate fixation in cervical laminoplasty compared to continuous fixation.
J Orthop Surg Res
January 2025
Department of Orthopedic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 Huaihai Road, Xuzhou, Jiangsu, 221002, China.
Purpose: To compare the efficacy and safety of skip titanium plates combined with adjacent spinous process suture suspension versus continuous titanium plate fixation in cervical laminoplasty.
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Triglyceride-glucose index in predicting the risk of new-onset diabetes in the general population aged 45 years and older: a national prospective cohort study.
BMC Endocr Disord
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Department of Endocrinology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, 110033, China.
Objective: Insulin resistance (IR) is often present in diabetes, which imposes a heavy burden on the prevention and treatment of diabetes. Triglyceride glucose index (TyG) is simple, reliable and reproducible in detecting IR, and has great advantages in predicting the risk of diabetes. The aim of this study was to analyze the potential association between TyG and the risk of diabetes in Chinese middle-aged and older adults using a prospective cohort study design.
View Article and Find Full Text PDFNLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing.
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Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.
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View Article and Find Full Text PDFDevelopment and validation of a prognostic nomogram for predicting of patients with acute sedative-hypnotic overdose admitted to the intensive care unit.
Sci Rep
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Emergency Medicine Laboratory and the Department of Emergency, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, 610041, Sichuan, China.
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