AI Article Synopsis
- MCT-4 plays a critical role in transporting lactate in human cancers, particularly linked to non-small cell lung cancer, but its significance in lung adenocarcinoma (AC) was previously unclear.
- A study analyzed MCT-4 expression in 146 lung AC samples, finding that 17.1% exhibited high levels, which were notably associated with deeper tumor invasion and significantly poorer overall survival rates.
- High MCT-4 expression emerged as an independent prognostic factor for lung AC patients, suggesting it could be a potential target for treatment.
Article Abstract
Monocarboxylate transporter 4 (MCT-4) serves a key function in transporting lactate across the plasma membrane in various types of human cancer. Evidence indicates that MCT-4 expression is associated with non-small cell lung cancer; however, the distribution and clinical significance of MCT-4 in the lung adenocarcinoma (AC) subtype remain unknown. Thus, the aim of the present study was to explore the clinicopathological significance and prognostic values of MCT-4 expression in lung AC. Quantum dots-based immunofluorescence histochemistry was performed to observe the expression of MCT-4 in 146 specimens of lung AC and corresponding normal lung tissues. MCT-4 protein and mRNA were detected by western blotting and reverse transcription-quantitative polymerase chain reaction from 30 fresh samples of lung AC and corresponding normal lung tissues. Of the 146 samples, 25 (17.1%) exhibited high and 121 (82.9%) exhibited low MCT-4 expression. MCT-4, at the protein and mRNA level, was significantly increased in tumor specimens compared with corresponding normal lung tissue (P<0.05). MCT-4 protein expression was significantly associated with depth of invasion (P=0.034). A survival curve analysis indicated that high MCT-4 expression in lung AC was associated with a decreased overall survival rate (P=0.001). Multivariate analysis demonstrated that high MCT-4 level was an independent prognostic factor (hazard ratio, 3.192; 95% confidence interval, 1.804-5.646; P=0.001) for patients with lung AC. The results have demonstrated that high MCT-4 expression is significantly associated with the poor prognosis and disease progression of patients with lung AC. Therefore, MCT-4 may be a candidate therapeutic target in lung AC.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661367 | PMC |
| http://dx.doi.org/10.3892/ol.2017.6964 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization and comparison of hypoxia inducing factors on tumor growth and metastasis between two- and three-dimensional cancer models.
SLAS Discov
October 2023
ImmunityBio, Culver City, CA 90232, USA. Electronic address:
The monocarboxylic acid transporter 4 (Mct-4), a downstream biomarker of hypoxia inducing factor (HIF)-1α, is involved in the cellular response to hypoxia, as indicated by the hypoxic response element in its promoter region. Using a tumorsphere assay as an in vitro 3-dimensional (3D) model generated using 384-well ultra-low attachment (ULA) plates for cell proliferation analysis using a plate-based image cytometer, we identify a hypoxic response in the tumorsphere model that is distinct from that of cells grown under 2-dimensional (2D) normoxic conditions and demonstrate a key role for Mct-4 in enabling 3D growth. The tumorsphere model yields evidence of an essential role for Mct-4 in multiple cell lines, which were genetically modified to underexpress and overexpress Mct-4, evidence not apparent in a standard 2D model of growth in the same cell lines.
View Article and Find Full Text PDFHarnessing the Power of Sugar-Based Nanoparticles: A Drug-Free Approach to Enhance Immune Checkpoint Inhibition against Glioblastoma and Pancreatic Cancer.
ACS Nano
October 2024
Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan, R.O.C.
Article Synopsis
- Cancer cells have a high demand for sugars, and researchers developed glycopolymer-like nanoparticles that target specific receptors on cancer-associated macrophages to enhance their effectiveness in fighting tumors.
- These nanoparticles, created through a carbonization process, can alter immune cell polarization and cellular metabolism, promoting a transition from M2 (immunosuppressive) to M1 (pro-inflammatory) macrophages, which helps overcome tumor defenses.
- In models of glioblastoma and pancreatic cancer, these nanoparticles not only induced cancer cell death but also improved the effectiveness of immunotherapy by remodeling the tumor microenvironment, activating immune responses, and facilitating the activity of cytotoxic T lymphocytes and dendritic cells.
Glucose metabolism transcriptome clustering identifies subsets of resectable lung adenocarcinoma with different prognoses.
JTCVS Open
August 2024
Thoracic Surgery Department, Cochin Hospital, Centre Université de Paris, Paris University, Paris, France.
Objectives: Reprogramming of energy metabolism is a well-established hallmark of cancer, with aerobic glycolysis classically considered a prominent feature. We investigate the heterogeneity in glucose metabolism pathways within resectable primary lung adenocarcinoma and its clinical significance.
Methods: Using The Cancer Genome Atlas data, RNA expressions were extracted from 489 primary lung adenocarcinoma samples.
The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways.
Reprod Sci
August 2024
Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
Article Synopsis
- The study investigated how a high-fat diet (HFD) affects metabolic disorders in the testicles of male rats and whether different exercise modalities can help counteract these effects.
- Thirty mature male Wistar rats were divided into groups to test the effects of HFD alone and various exercise intensities, including moderate and high-intensity workouts.
- Results showed that rats on HFD alone had reduced testicular function, but those who exercised, especially with high-intensity interval training (HIIT), showed improvements in key metabolic markers and supported better male fertility outcomes.
MCT4-driven CAF-mediated metabolic reprogramming in breast cancer microenvironment is a vulnerability targetable by miR-425-5p.
Cell Death Discov
March 2024
Department of Molecular Medicine and Medical Biotechnology, "Federico II" University of Naples, Naples, Italy.
Multiple oncogenic alterations contribute to breast cancer development. Metabolic reprogramming, deeply contributing to tumor microenvironment (TME) education, is now widely recognized as a hallmark of cancer. The reverse Warburg effect induces cancer-associated fibroblasts (CAFs) to produce and secrete L-lactate, enhancing malignant characteristics such as neoangiogenesis, metastatic dissemination, and treatment resistance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!