Microspectrophotometric DNA measurements of carcinoma-in-situ germ cells in the testis.

Early detection of premalignant changes of the testis in at-risk groups seems to be of considerable significance. Hitherto the diagnosis of carcinoma-in-situ testis has been based upon conventional histological techniques. A microspec-trophotometric study of the DNA content in Feulgen stained carcinoma-in-situ germ cells of eight infertile men revealed an aneuploid DNA distribution pattern. The results support the assumption that these cells are of malignant nature, and furthermore, the findings indicate that DNA measurements may be of value in discriminating between malignant and non-malignant intra-tubular germ cells in testicular biopsies.