AI Article Synopsis
- This research presents a new method for biosensor design that combines the processes of surface immobilization and bioconjugation into one step, inspired by how mussels adhere to surfaces.
- By using dopamine or catechol in polydiacetylene (PDA) liposomes, researchers achieved effective attachment to various substrates without needing to alter those surfaces.
- The method was successfully applied in a droplet-array biosensor, allowing for the sensitive detection of vascular endothelial growth factor at very low concentrations (10 nM).
Article Abstract
Most solid-state biosensor platforms require a specific immobilization chemistry and a bioconjugation strategy separately to tether sensory molecules to a substrate and attach specific receptors to the sensory unit, respectively. We developed a mussel-inspired universal conjugation method that enables both surface immobilization and bioconjugation at the same time. By incorporating dopamine or catechol moiety into self-signaling polydiacetylene (PDA) liposomes, we demonstrated efficient immobilization of the PDA liposomes to a wide range of substrates, without any substrate modification. Moreover, receptor molecules having a specificity toward a target molecule can also be attached to the immobilized PDA liposome layer without any chemical modification. We applied our mussel-inspired conjugation method to a droplet-array biosensor by exploiting the hydrophilic nature of PDA liposomes coated on a hydrophobic polytetrafluoroethylene surface and demonstrated selective and sensitive detection of vascular endothelial growth factor down to 10 nM.
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| http://dx.doi.org/10.1021/acsami.7b14086 | DOI Listing |
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