AI Article Synopsis
- The study aimed to create a protocol for MEGA-PRESS spectroscopy to detect GABA levels in the mouse brain at 9.4 T and assess the impact of isoflurane anesthesia on these levels.
- Implementation of the MEGA-PRESS sequence involved conducting multiple scans on anesthetized male mice, with a focus on the thalamus, and utilizing an automated analysis program to enhance data accuracy.
- Results indicated a significant decrease in GABA levels relative to creatine over the 2-hour anesthesia period, confirming MEGA-PRESS as an effective method for in vivo GABA measurement in mouse brains.
Article Abstract
The goals of this study were to develop an acquisition protocol and the analysis tools for Meshcher-Garwood point-resolved spectroscopy (MEGA-PRESS) in mouse brain at 9.4 T, to allow the in vivo detection of γ-aminobutyric acid (GABA) and to examine whether isoflurane alters GABA levels in the thalamus during anesthesia. We implemented the MEGA-PRESS sequence on a Bruker 94/20 system with ParaVision 6.0.1, and magnetic resonance spectra were acquired from nine male wild-type C57BL/6 J mice at the thalamus. Four individual scans were obtained for each mouse in a 2-h time course whilst the mouse was anesthetized with isoflurane. We developed an automated analysis program with improved correction for frequency and phase drift compared with the standard creatine (Cr) fitting-based method and provided automatic quantification. During MEGA-PRESS acquisition, a single voxel with a size of 5 × 3 × 3 mm was placed at the thalamus to evaluate GABA to Cr (GABA/Cr) ratios during anesthesia. Detection and quantitative analysis of thalamic GABA levels were successfully achieved. We noticed a significant decrease in GABA/Cr during the 2-h anesthesia (by linear regression analysis: slope < 0, p < 0.0001). In summary, our findings demonstrate that MEGA-PRESS is a feasible technique to measure in vivo GABA levels in the mouse brain at 9.4 T.
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| http://dx.doi.org/10.1002/nbm.3837 | DOI Listing |
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