Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of Hsp90-Cdc37-Cdk4 kinase protein complex from utilizing the viral 2A sequences to titrate the three proteins at similar levels.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669387 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2563 | DOI Listing |
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