AI Article Synopsis
- Conjunctival injuries pose significant treatment challenges, and a promising solution is using biodegradable scaffolds to deliver conjunctival epithelial cells for repair.
- An ultrathin porous nanofibrous scaffold made from collagen and PLCL was created, measuring just 20 µm thick with high porosity, allowing for effective cell proliferation and viability.
- The study found that cells grown on these scaffolds expressed specific conjunctival genes and showed no significant inflammatory response, indicating their potential for repairing conjunctival epithelial coloboma through observed stratification of cell layers after two weeks.
Article Abstract
Conjunctival injuries are general but intractable ocular surface diseases, the sequelae of which are particularly challenging to treat. A promising therapy for conjunctival injuries is to employ biodegradable scaffolds to deliver conjunctival epithelial cells for repairing damaged or diseased conjunctiva. In the present study, an ultrathin porous nanofibrous scaffold was fabricated by using collagen and poly(L-lactic acid-co-ε-caprolactone) (PLCL) and displayed a thickness of 20 µm, with a high porosity and an average fiber diameter of 248.83±26.44 nm. Conjunctival epithelial cells seeded on the scaffolds proliferated well and had a high cell viability. Reverse-transcription quantitative PCR showed the expression of conjunctival epithelial cell-specific genes; in addition, there was no significant difference in the inflammatory gene expression between cells grown on collagen/PLCL scaffolds and tricalcium phosphate scaffolds. After co-culture for 2 weeks , epithelial cell stratification was observed using hematoxylin and eosin staining, exhibiting three to four epithelial-cell layers. In conclusion, these results suggested that collagen/PLCL scaffolds have potential application for repairing conjunctival epithelial coloboma.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658689 | PMC |
| http://dx.doi.org/10.3892/etm.2017.5073 | DOI Listing |
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