Objective: To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens.
Results: 266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest β-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P , a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P improved β-Gal activity by ~ 200%.
Conclusion: A new strong promoter for protein expression and genetic engineering of Bacillus species.
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| http://dx.doi.org/10.1007/s10529-017-2449-4 | DOI Listing |
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