AI Article Synopsis
- Mannosylation in the endoplasmic reticulum is essential for synthesizing glycans using donor substrates like GDP-Man and dolichol phosphate-mannose.
- Recent research reveals that low-abundant uridine diphosphate-mannose (UDP-Man) exists in mammals, challenging previous assumptions about glycan synthesis.
- High concentrations of UDP-Man were found in mouse brain regions, and its levels increased in human cell lines upon mannose supplementation, suggesting its regulatory role in glycosylation in specific tissues.
Article Abstract
Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
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| http://dx.doi.org/10.1016/j.bbrc.2017.10.173 | DOI Listing |
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