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Introducing the new bacterial branch of the RNase A superfamily. | LitMetric

Introducing the new bacterial branch of the RNase A superfamily.

RNA Biol

a Department of Molecular Biology and Biochemistry , University of California Irvine, Irvine , CA , USA.

Published: January 2018

AI Article Synopsis

Article Abstract

Bovine pancreatic ribonuclease (RNase A) is the founding member of the RNase A superfamily. Members of this superfamily of ribonucleases have high sequence diversity, but possess a similar structural fold, together with a conserved His-Lys-His catalytic triad and structural disulfide bonds. Until recently, RNase A proteins had exclusively been identified in eukaryotes within vertebrae. Here, we discuss the discovery by Batot et al. of a bacterial RNase A superfamily member, CdiA-CT: a toxin that belongs to an inter-bacterial competition system from Yersinia kristensenii. CdiA-CT exhibits the same structural fold as conventional RNase A family members and displays in vitro and in vivo ribonuclease activity. However, CdiA-CT shares little to no sequence similarity with RNase A, and lacks the conserved disulfide bonds and catalytic triad of RNase A. Interestingly, the CdiA-CT active site more closely resembles the active site composition of various eukaryotic endonucleases. Despite lacking sequence similarity to eukaryotic RNase A family members, CdiA-CT does share high sequence similarity with numerous Gram-negative and Gram-positive bacterial proteins/domains. Nearly all of these bacterial homologs are toxins associated with virulence and bacterial competition, suggesting that the RNase A superfamily has a distinct bacterial subfamily branch, which likely arose by way of convergent evolution. Finally, RNase A interacts directly with the immunity protein of CdiA-CT, thus the cognate immunity protein for the bacterial RNase A member could be engineered as a new eukaryotic RNase A inhibitor.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786019PMC
http://dx.doi.org/10.1080/15476286.2017.1387710DOI Listing

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