We report on the first experimental realization of the controlled-not (cnot) quantum gate and entanglement for two individual atoms of different isotopes and demonstrate a negligible cross talk between two atom qubits. The experiment is based on a strong Rydberg blockade for ^{87}Rb and ^{85}Rb atoms confined in two single-atom optical traps separated by 3.8 μm. The raw fidelities of the cnot gate and entanglement are 0.73±0.01 and 0.59±0.03, respectively, without any corrections for atom loss or trace loss. Our work has applications for simulations of many-body systems with multispecies interactions, for quantum computing, and for quantum metrology.
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