MicroRNA (miRNA) is a non-protein-coding small RNA molecule that negatively regulates gene expression by degradation of mRNA or suppression of mRNA translation. MiRNAs play important roles in biological processes such as cellular development, differentiation, proliferation, apoptosis and stem cell self-renewal and cancer development. The expression profile of microRNAs is tissue-, cell-type specific. PCR- and microarray-based arrays are the commonly used for differential expression of microRNAs between different diseased conditions. With the next-generation sequencing or massively parallel DNA sequencing technology advanced and the cost is plummeting, microRNAseq approach is widely used for the genome-wide discovery of known and unknown miRNA expression. However, the analysis of microRNAseq is computational expensive, here I provide guidelines and available tools for microRNAseq analysis.
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CLDN4 belongs to a multi-transmembrane protein family of claudins, which mainly functions in cell-cell adhesion and migration. MicroRNAs (miRNAs) are important post-transcriptional regulating factors that participate in broad biological process including immunity. Through high-throughput RNA sequencing strategy, a flounder miRNA, miR-29-x, was identified to be responsible to both bacteria and virus.
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