Redundant CArG Box -motif Activity Mediates Transcriptional Regulation during Gynoecium Development.

Front Plant Sci

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States.

Published: October 2017

AI Article Synopsis

  • The development of the dehiscence zone (DZ) in seed pods is influenced by specific genes controlled by transcription factors FRUITFULL (FUL) and REPLUMLESS (RPL), which restrict gene expression to the gynoecial valve margin (VM).
  • The study identifies distinct regulatory regions - a 1kb promoter and a 700bp enhancer - within a 2.2kb area upstream of a key gene responsible for VM and DZ expression.
  • Mutations of CArG box motifs in this region indicate their role in repressing gene expression in the valve, contributing to the fine regulation necessary for correct seed pod development.

Article Abstract

In the seed pod, pod shatter and seed dispersal properties are in part determined by the development of a longitudinally orientated dehiscence zone (DZ) that derives from cells of the gynoecial valve margin (VM). Transcriptional regulation of the MADS protein encoding transcription factors genes () and () are critical for proper VM identity specification and later on for DZ development. Current models of and regulation indicate that the transcription factors FRUITFULL (FUL) and REPLUMLESS (RPL) repress these genes in the developing valve and replum domains, respectively. Thus the expression of the genes is restricted to the VM. encodes a MADS-box containing transcription factor that is predicted to act through CArG-box containing -regulatory motifs. Here we delimit functional modules within the cis-regulatory region and examine the functional importance of CArG box motifs within these regulatory regions. We have characterized a 2.2kb region upstream of the translation start site that drives early and late medial domain expression in the gynoecium, as well as expression within the VM and DZ. We identified two separable, independent -regulatory modules, a 1kb promoter region and a 700bp enhancer region, that are capable of giving VM and DZ expression. Our results argue for multiple independent -regulatory modules that support expression during VM development and may contribute to the robustness of expression in this tissue. Additionally, three closely positioned CArG box motifs located in the upstream regulatory region were mutated in the context of the 2.2kb reporter construct. Mutating simultaneously all three CArG boxes caused a moderate de-repression of the reporter that was detected within the valve domain, suggesting that these CArG boxes are involved in repression in the valve.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650620PMC
http://dx.doi.org/10.3389/fpls.2017.01712DOI Listing

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