CRISPR/Cas9-mediated gene editing may involve nonhomologous end-joining to create various insertion/deletions (indels) or may employ homologous recombination to modify precisely the target DNA sequence. Our understanding of these processes has been guided by earlier studies using other site-specific endonucleases, both in model organisms such as budding yeast and in mammalian cells. We briefly review what has been gleaned from such studies using the HO and I-SceI endonucleases and how these findings guide current gene editing strategies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835394 | PMC |
| http://dx.doi.org/10.1021/acschembio.7b00760 | DOI Listing |
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