In this study, the indigenous microbial mineralisation of C-phenanthrene in seven background soils (four from Norwegian woodland and three from the UK (two grasslands and one woodland)) was investigated. ∑PAHs ranged from 16.39 to 285.54 ng g dw soil. Lag phases (time before C-phenanthrene mineralisation reached 5%) were longer in all of the Norwegian soils and correlated positively with TOC, but negatively with ∑PAHs and phenanthrene degraders for all soils. C-phenanthrene mineralisation in the soils varied due to physicochemical properties. The results show that indigenous microorganisms can adapt to C-phenanthrene mineralisation following diffuse PAH contamination. Considering the potential of soil as a secondary PAH source, these findings highlight the important role of indigenous microflora in the processing of PAHs in the environment.
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http://dx.doi.org/10.1039/c7em00242d | DOI Listing |
Microbiol Res
January 2023
Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK.
In this study, the biodegradation of phenanthrene was investigated in newly isolated endophytic fungal strains, Fusarium sp. (KTS01), Trichoderma harzianum (LAN03), Fusarium oxysporum (KTS02), Fusarium oxysporum (LAN04), and Clonostachys rosea (KTS05). This was performed under different carbon:nitrogen ratios (10:1, 20:1, and 30:1) using different nitrogen sources (urea and malt extract and ammonium nitrate) over a 30 d incubation period in both static and agitated liquid media.
View Article and Find Full Text PDFSci Total Environ
March 2022
Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK. Electronic address:
In this study, an investigation was carried out to explore the the impact of white-rot fungi (WRF) on enhancing the development of phenanthrene catabolism in soil over time (1, 25, 50, 75 and 100 d). The WRF were immobilised on spent brewery grains (SBG) prior to inoculation to the soil. The results showed that SBG-immobilised WRF-amended soils reduced the lag phases and increased the extents of C-phenanthrene mineralisation.
View Article and Find Full Text PDFEnviron Pollut
March 2021
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada; NRC-University of Alberta Nanotechnology Initiative, Nanotechnology Research Centre, Edmonton, Canada.
Hydraulic fracturing has become widely used in recent years to access vast global unconventional sources of oil and gas. This process involves the injection of proprietary mixtures of water and chemicals to fracture shale formations and extract the hydrocarbons trapped within. These injection fluids, along with minerals, hydrocarbons, and saline waters present within the formations being drilled into, return to the surface as flowback and produced water (FPW).
View Article and Find Full Text PDFEcotoxicol Environ Saf
June 2020
Lancaster Environment Centre, Lancaster University, UK.
The impact of whole digestate (WD) and its fractions (solid [SD] and liquid [LD]) on C-phenanthrene mineralization in soil over 90 d contact time was investigated. The C-phenanthrene spiked soil was aged for 1, 30, 60 and 90 d. Analysis of water-soluble nitrogen, phosphorus, total (organic and inorganic) carbon, and quantitative bacterial count were conducted at each time point to assess their impact on mineralization of C-phenanthrene in soils.
View Article and Find Full Text PDFWhen soil is frozen, biochar promotes petroleum hydrocarbon (PHC) degradation, yet we still do not understand why. To investigate microbial biodegradation activity under frozen conditions, we placed 60-μm mesh bags containing 6% (v/v) biochar created from fishmeal, bonemeal, bone chip, or wood into PHC-contaminated soil, which was then frozen to -5°C. This created three soil niches: biochar particles, the charosphere (biochar-contiguous soil), and bulk soil outside of the bags.
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