Construction of chimaeric MVA is a useful tool with which to study gene function of related viruses. The protocol given here describes MVA chimaeras containing genes from Fowlpox virus (FWPV), although this can be applied to DNA derived from other organisms. There are a number of steps required to make the chimaeric MVA: 1) Purification of viral particles; 2) Extraction of DNA from purified viral particles; 3) Assembly of linear recombination templates; 4) Transfection of linear recombination templates; 5) Selection of chimaeric MVA. .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659373 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.1376 | DOI Listing |
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