A stability-indicating isocratic reversed-phase ion-pair chromatographic method was designed for the separation of impurities in the presence of degradation products. Marbofloxacin tablets and a placebo were exposed to the stress conditions of oxidative, acid, base, humidity, thermal, and photolytic degradation. Significant and moderate degradation was observed in acidic and oxidative stress conditions, respectively. The degradation products were well resolved from the main peak and its impurities, thus proving the stability-indicating analytical method. The method was developed by using an XTerra RP18 3.5 μm (150 × 4.6 mm) column, with the mobile phase containing a mixture of buffer (pH 2.5)-methanol-glacial acetic acid (77 + 23 + 0.5, v/v). The flow rate of the mobile phase was 1.2 mL/min, with a column oven temperature of 40°C and a detection wavelength of 315 nm. The proposed method met Veterinary International Conference on Harmonization requirements and was successfully used for impurity quantitation in marbofloxacin tablets.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5740/jaoacint.17-0108 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Green Method for Evaluation of Marbofloxacin Tablets by HPLC and Evaluation of Interchangeability With UV and Turbidimetric Methods.
J AOAC Int
November 2023
Federal University of Goiás, Faculty of Pharmacy, Laboratório de Controle de Qualidade, Rua 240, s/n, 74605-170, Goiânia, Goiás, Brazil.
Background: Marbofloxacin (MAR) is a veterinary antimicrobial, marketed in injectable solution, oral suspension, and tablets. MAR has no monograph for tablet evaluation in official compendiums. High Performance Liquid Chromatography (HPLC) methods present in the literature for evaluating MAR in tablets do not follow the principles of green and sustainable analytical chemistry.
View Article and Find Full Text PDFDomestic Waste and Wastewaters as Potential Sources of Pharmaceuticals in Nestling White Storks ().
Antibiotics (Basel)
March 2023
Toxicology and Risk Assessment Group, Biomedical Research Institute of Murcia (IMIB-Arrixaca), University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Information on the exposure of wild birds to pharmaceuticals from wastewater and urban refuse is scarce despite the enormous amount of drugs consumed and discarded by human populations. We tested for the presence of a battery of antibiotics, NSAIDs, and analgesics in the blood of white stork () nestlings in the vicinity of urban waste dumps and contaminated rivers in Madrid, central Spain. We also carried out a literature review on the occurrence and concentration of the tested compounds in other wild bird species to further evaluate possible shared exposure routes with white storks.
View Article and Find Full Text PDFFast and New Microbiological Method for Evaluating the Potency of Marbofloxacin-Based Tablets.
J AOAC Int
May 2023
Universidade Federal de Goiás-UFG, Faculdade de Farmácia, Laboratório de Controle de Qualidade, Goiânia, Goiás, Brazil.
Background: Marbofloxacin (MAR) is an antimicrobial belonging to the fluoroquinolone class and is marketed in the form of tablets. There is no microbiological monograph in official compendia to evaluate the potency of the final product. Furthermore, the literature is scarce in this context.
View Article and Find Full Text PDFEco-Friendly UV Spectrophotometric Method for Evaluation of Marbofloxacin in Tablets: Stability Study.
J AOAC Int
June 2022
Laboratório de Controle de Qualidade, Faculdade de Farmácia, Universidade Federal de Goiás-UFG, Street 240, Goiânia, Goiás 74605-170, Brazil.
Background: Marbofloxacin (MAR) is an antimicrobial for veterinary use, and it does not have a monograph in an official compendium for the pharmaceutical form in tablets.
Objective: In this context, the objective of this work was to develop and validate an effective, eco-friendly, and indicative of stability method by spectrophotometry in the ultraviolet region (UV) for quantitative evaluation of MAR in tablets.
Method: Purified water-ethanol (80:20, v/v) as a diluent, quartz cubette and 296 nm were used.
An Overview of Analytical Methods for the Quantification of Marbofloxacin in Pharmaceutical, Biological, and Food Matrixes.
J AOAC Int
March 2022
Federal University of Goiás, Faculty of Pharmacy, Street 240, Goiânia, Goiás 74605-170, Brazil.
Background: Marbofloxacin (MAR), a second-generation fluoroquinolone, is used in veterinary medicine in the form of tablets. It has a broad spectrum of action, low toxicity, and limited development of bacterial resistance. The analytical methods available in the literature become more important since MAR in tablets does not have a monograph in official compendiums.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!