12/15-Lipoxygenase (12/15-LOX) contributes to the brain damage after middle cerebral artery occlusion (MCAO) in the acute phase of stroke. The aim of this study was to investigate the effects of a 12/15-LOX inhibitor, LOXBlock-1(LB1), in mice using a FeCl-induced permanent distal MCAO model and FeCl-induced ischemia/thrombolysis with tPA. In order to induce permanent distal MCAO, 30% FeCl was used in C57BL6 mice. LB1 or DMSO treatments were applied intraperitoneally 2 h following MCAO. For FeCl-induced ischemia/thrombolysis experiments, 10% FeCl was preferred so as to obtain reperfusion with tPA in CD1 mice. 4 h following ischemia either LB1 or DMSO and iv tPA was administered. Outcomes were NSS, weight loss, infarct volume, hemorrhage area and reperfusion rate. FeCl-induced distal MCAO caused an increase in 12/15-LOX signal in the ischemic cortex with an increase in MDA2 and AIF immunoreactivity. LB1 treatment, applied 2 h after ischemia, significantly decreased the infarct volume at 24 h of permanent distal MCAO. Weight loss was also significantly reduced in LB1 treated group. Distal MCAO and tPA application with LB1 or DMSO showed that treatment significantly decreased the infarct volume and the hemorrhage area. The reperfusion rate in the LB1-treated group was surprisingly higher than in the DMSO group and NSS results were significantly improved. These data suggest that LB1 can be used as an adjuvant agent to tPA. This study not only shows the effects of LB1 treatment in distal MCAO but also confirms that FeCl-induced MCAO model can be a useful tool to screen novel treatment options in stroke.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5714685 | PMC |
| http://dx.doi.org/10.1016/j.brainres.2017.10.024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Method for Imaging the Ischemic Penumbra with MRI using IVIM.
AJNR Am J Neuroradiol
January 2025
From the Department of Radiology, Medical Physics (MML, TJC), Department of Interventional Radiology (NS, GAC), Department of Surgery and Large Animal Studies (MAN), and the Department of Statistics (MG), University of Chicago, Chicago, IL, USA; Department of Anesthesiology (SPR), University of Illinois, Chicago, IL, USA; Department of Radiology (MSS), University of Massachusetts Chan Medical School, Worcester, MA, USA; Department of Radiology, Biomedical Engineering and Imaging Institute (Current affiliation MML), Icahn School of Medicine at Mount Sinai, New York, NY, USA; Mount Carmel Health Systems (Current affiliation GAC), Columbus, OH, USA.
Background And Purpose: In acute ischemic stroke, the amount of "local" CBF distal to the occlusion, i.e. all blood flow within a region whether supplied antegrade or delayed and dispersed through the collateral network, may contain valuable information regarding infarct growth rate and treatment response.
View Article and Find Full Text PDFVX-765 attenuates secondary damage and β-amyloid accumulation in ipsilateral thalamus after experimental stroke in rats.
Exp Neurol
December 2024
Department of Neurology and Stroke Center, The First Affiliated Hospital with Jinan University, Guangzhou, China. Electronic address:
Focal cortical infarction can result both in the accumulation of Aβ in as well as further secondary damage and inflammation within the ipsilateral thalamus. VX-765 is a potent and selective small-molecule capable of inhibiting caspase-1, which has been shown to exhibit active neuroprotection properties in multiple disease. However, the neuroprotection efficacy of VX-765 as a means of attenuating secondary damage after MCAO remains uncertain.
View Article and Find Full Text PDFAnisodine hydrobromide injection promotes neural remodeling and recovery after ischemic stroke in mice.
Gen Physiol Biophys
November 2024
Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Article Synopsis
- * The study tested anisodine hydrobromide on healthy male mice with induced middle cerebral artery occlusion, assessing its impact on neural growth and recovery using various staining techniques.
- * Results indicated that anisodine hydrobromide enhances nerve regeneration and synaptic formation, while the Notch signaling pathway plays a crucial role in its effects on neuroplasticity post-stroke.
A new angiographic scoring for grading the difficulty of recanalization for symptomatic non-acute middle cerebral artery occlusions.
Front Neurosci
October 2024
Department of Neurosurgery, The Third Affiliated Hospital of Soochow University, Changzhou, China.
Background: Endovascular recanalization is a feasible option for treating symptomatic non-acute middle cerebral artery occlusion (MCAO) patients. Hence, we aimed to establish a new angiographic scoring to grade the recanalization difficulty of MCAO to determine the suitable patients for endovascular treatment.
Methods: We retrospectively analyzed a total of 113 consecutive recurrent symptomatic non-acute MCAO patients who underwent endovascular recanalization from July 2015 to August 2021 in four Chinese comprehensive stroke centers.
Extracellular Vesicles Obtained from Hypoxic Mesenchymal Stromal Cells Induce Neurological Recovery, Anti-inflammation, and Brain Remodeling After Distal Middle Cerebral Artery Occlusion in Rats.
Transl Stroke Res
September 2024
Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Article Synopsis
- Small extracellular vesicles (sEVs) from mesenchymal stromal cells (MSCs) show promise for stroke treatment by enhancing brain tissue survival and promoting angiogenesis, especially when the MSCs are cultured in hypoxic conditions.
- In a study involving rats with permanent distal MCAO, sEVs from hypoxic MSCs improved motor-coordination recovery and reduced brain inflammation compared to untreated controls.
- Although hypoxic MSC-sEVs did not affect infarct volume, they significantly increased proliferating endothelial cells, suggesting they have a positive impact on recovery and healing in stroke models.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!