AI Article Synopsis
- Ion Torrent next-generation sequencing (NGS) technology was used to study how acyclovir-resistant herpes simplex virus type 1 (HSV-1) develops in patients who had hematopoietic stem cell transplants.
- The NGS method not only confirmed all mutations found through traditional Sanger sequencing but also detected additional mutations that confer resistance to acyclovir.
- This new approach provides a more detailed understanding of the emergence of these mutations and allows for earlier diagnosis of acyclovir-resistant HSV-1 infections compared to the traditional sequencing method.
Article Abstract
Ion Torrent next-generation sequencing (NGS) technology was applied to study the mode of emergence of acyclovir (ACV)-resistant (ACVr) herpes simplex virus type 1 (HSV-1) in patients with hematopoietic stem cell transplantation (HSCT) by quantitatively detecting mutations in the viral thymidine kinase (vTK) gene in the HSV-1 isolates recovered from HSCT patients. All of the mutations detected with the Sanger sequencing method in the vTK genes of HSV-1 isolates were also detected with the NGS assay. Furthermore, different mutations, which conferred ACV resistance and were not detected with the Sanger sequencing method, were also detected in a quantitative manner by using the NGS assay. The approach described here is applicable to studying the emergence process of vTK gene mutation-associated ACVr HSV-1 more in detail than the Sanger method. The NGS assay makes it possible to make a diagnosis of vTK gene mutation-associated ACVr HSV-1 infections at the early stage, which the ratio of ACVr HSV-1 is much lower than that of ACV-sensitive (ACVs) HSV-1.
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| http://dx.doi.org/10.1016/j.jviromet.2017.10.019 | DOI Listing |
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