[Comparison of Protective Effect of Electroacupuncture on Myocardial Ischemia Injury Between Different Acupoint Formulas in Rats].

Objective: To observe the different effects of electroacupucnture of "Neiguan" (PC 6) grouping with other acupoints on myocardial ischemia (MI) injury, so as to provide evidence for clinical acupoint formulas.

Methods: Forty Wistar male rats were randomly divided into normal, model, EA 1[bilateral "Neiguan" (PC 6), bilateral "Zusanli" (ST 36),"Guanyuan" (CV 4)], and EA 2[bilateral "Neiguan" (PC 6), bilateral "Shenmen" (HT 7), "Danzhong" (CV 17)] groups, 10 rats in each group. The MI model was established by subcutaneous injection with ISO solution(5 mL/kg), once per day for 7 days. Both EA 1 and EA 2 groups were subjected to EA (2 Hz/100 Hz, 1 mA) for 10 min before subcutaneous injection, once daily for 21 days. Electrocardiogram (ECG) was recorded after treatment. Serum cTnT was detected by biochemistry. SOD, GSH-Px and MDA in myocardial tissues of left ventricular were measured by ELISA, and the ultrastructural changes of myocardial cells in the left ventricular were observed by electron microscope.

Results: Compared with the normal group, the amplitude of S-T segment and HR of ECG, cTnT and MDA contents were significantly increased in the model group (<0.01) accompanied with seriously destroyed ultrastructure in the myocardial tissue, while SOD and GSH-Px levels were lower than those in the normal group (<0.01). However, EA treatments reversed all the abnormal changes in the model group (<0.05). There was no significant difference between EA 1 and EA 2 groups in the amplitude of S-T segment and HR (>0.05); cTnT and MDA levels in the EA 1 group were lower than those in the EA 2 group (<0.05), while SOD and GSH-Px levels were higher than those in the EA 2 group (<0.05), accompanied with lighter degree of ultrastructure damage in the myocardial tissue in the EA 1 group.

Conclusions: EA can prevent myocardial ischemic injury, and the effect of EA 1 treatment was better than that of EA 2.