Xanthine oxidase (XO) found in all mammals and excess activity leads to urolithiasis. The cow milk XO was purified to 305-fold with a specific activity of 8.76 EU/mg and overall yield of 87% by using DEAE-Sepharose chromatography. The phenolics showed potent XO inhibitory effect with K , P1 (0.412), P2 (0.632), P3 (0.585), P4 (0.886), P5 (1.633), P6 (0.503), P7 (2.882), P8 (3.761), P9 (4.487), and P10 (5.841) μM. The phenolics P9 and P10 exhibited uncompetitive inhibition; the phenolics P1, P2, P3, P4, and P6 showed competitive inhibition, and other phenolic acids showed noncompetitive inhibition. The studied phenolic compounds showed potent antioxidant activity and expressed as EC , ranged from, DPPH (4.2-25.8 μg mL ), ABTS (10.2-42.5 mmol TE 100 g ), and FRAP (6.3-36.8 mol Fe (II) 100 g ). The results obtained from this study might be utilized for design of XO inhibitors and as antigout agent.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbt.22005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Serological, phenotypic and molecular characterization of brucellosis in small ruminants in northern Algeria.
Front Microbiol
January 2025
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
Brucellosis is considered a common bacterial zoonotic disease of high prevalence in countries of the Middle East and the Mediterranean region with economic and public health impact. The present study aimed to investigate the current situation of brucellosis in small ruminants reared in Médéa and Sidi Bel-Abbès provinces, north Algeria. To achieve this objective, 96 sera (77 sheep and 19 goat) and 57 milk (42 sheep and 15 goat) samples were collected from suspected infected animals and serologically analyzed by using ELISA.
View Article and Find Full Text PDFDetermining causal pathogens and inflammatory state of mastitis in dairy cows via Gram staining of precipitates in milk.
Front Vet Sci
January 2025
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashihiroshima, Hiroshima, Japan.
Early detection of bovine mastitis-causing pathogens is necessary for treatment. As culturing methods are time-consuming, a more rapid detection technique is required. This study investigated the sensitivity, specificity, and detection limit of Gram staining of milk precipitates (milk Gram stain, MGS) to detect bovine mastitis-causing pathogens in milk, as well as the potential of MGS to diagnose inflammation by counting polymorphonuclear leukocytes (PMN).
View Article and Find Full Text PDFBibliometric Analysis of Global Pediatric Research on Cow's Milk Protein Allergy.
J Asthma Allergy
January 2025
Department of Pediatric Gastroenterology, Fuzhou Children's Hospital of Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
Background: Cow's milk protein allergy (CMPA) is a prevalent food allergy in early childhood, significantly impacting the quality of life for affected children. Current palliative measures, such as specialized formula milk, offer temporary relief but are costly and fail to address the underlying issue. Thus, there is a critical need to better understand CMPA and explore new treatment options.
View Article and Find Full Text PDFAn Au-Ag@Au fiber surface plasmon resonance sensor for highly sensitive detection of fluoroquinolone residues.
Analyst
January 2025
Tianjin Key Laboratory for Marine Environmental Research and Service, School of Marine Science and Technology, Tianjin University, Tianjin 300072, PR China.
Antibiotic residue detection plays an important role in protecting human health, but real-time, rapid, and highly sensitive detection is still challenging. Herein, gold and silver nanoparticles (Au-Ag NPs) were grown on the surface of optical fibers and a 50 nm thick gold film was deposited on the sensor's surface to fabricate the Au-Ag@Au fiber SPR sensor. The sensitivity of the sensor reached 3512 nm per RIU in the refractive index range of 1.
View Article and Find Full Text PDFBreastfeeding with primary low milk supply: a phenomenological exploration of mothers' lived experiences of postnatal breastfeeding support.
Int Breastfeed J
January 2025
University College Dublin, Dublin, Ireland.
Background: Most women can produce enough milk to exclusively breastfeed. However, a small cohort are prevented from doing so due to a condition known as primary low milk supply. The aim of the study was to provide new insights into how mothers with this condition experience help and support from professionals, volunteer support groups, and partners.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!