Identification of differentially expressed genes in sunflower (Helianthus annuus) leaves and roots under drought stress by RNA sequencing.

Bot Stud

Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Xuefu Road 368, Harbin, 150086, People's Republic of China.

Published: October 2017

AI Article Synopsis

  • Sunflower is a crucial oil plant known for its drought resistance, and the study analyzed how its seedlings respond to drought stress by examining gene expression in leaves and roots after 24 hours of exposure.
  • 805 genes in leaves and 198 in roots were found to be differentially expressed due to drought, with more genes being up-regulated than down-regulated.
  • The findings contribute to our understanding of how sunflowers cope with drought and expand the knowledge of genes related to drought tolerance.

Article Abstract

Background: Sunflower is recognized as one of the most important oil plants with strong tolerance to drought in the world. In order to study the response mechanisms of sunflower plants to drought stress, gene expression profiling using high throughput sequencing was performed for seedling leaves and roots (sunflower inbred line R5) after 24 h of drought stress (15% PEG 6000). The transcriptome assembled using sequences of 12 samples was used as a reference.

Results: 805 and 198 genes were identified that were differentially expressed in leaves and roots, respectively. Another 71 genes were differentially expressed in both organs, in which more genes were up-regulated than down-regulated. In agreement with results obtained for other crops or from previous sunflower studies, we also observed that nine genes may be associated with the response of sunflower to drought.

Conclusions: The results of this study may provide new information regarding the sunflower drought response, as well as add to the number of known genes associated with drought tolerance.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656504PMC
http://dx.doi.org/10.1186/s40529-017-0197-3DOI Listing

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