AI Article Synopsis
- Auxin, a vital plant hormone, is primarily synthesized from tryptophan through enzymatic reactions involving TAA1 and YUCCA enzymes to produce indole-3-acetic acid (IAA).
- Researchers previously found that yucasin, an inhibitor of the YUC enzyme, had limited effects on wild-type plants but was effective in suppressing high-auxin phenotypes in YUC overexpression plants.
- A newly developed difluorinated yucasin analog, YDF, was identified as a potent inhibitor of YUC that can induce an auxin-deficient phenotype in Arabidopsis, showing promise for further studies in plant biology and agriculture.
Article Abstract
The plant hormone auxin plays a crucial role in plant growth and development. Indole-3-acetic acid (IAA), a natural auxin, is mainly biosynthesized by two sequential enzyme reactions catalyzed by TAA1 and YUCCA (YUC). TAA1 is involved in the conversion of tryptophan to IPA, and YUC catalyzes the conversion of IPA to IAA. We previously demonstrated that yucasin inhibits AtYUC1 enzyme activity and suppress high-auxin phenotype of YUC overexpression plants, although yucasin displayed weak effects on the auxin-related phenotype of wild-type plants. To develop more potent YUC inhibitors, various derivatives of yucasin were synthesized, and their structure-activity relationships were investigated. Yucasin difluorinated analog (YDF) (5-[2,6-difluorophenyl]-2,4-dihydro-[1,2,4]-triazole-3-thione) was identified to be a more potent YUC inhibitor than the original yucasin. YDF caused an auxin-deficient phenotype in Arabidopsis wild-type plants that was restored with auxin application. YDF was found to be highly stable regarding metabolic conversion in vivo, accounting for the potent activity of the inhibition of IAA biosynthesis in planta. Photoaffinity labeling experiments demonstrated that yucasin-type inhibitors bind to the active site of AtYUC1. YDF is a promising auxin biosynthesis inhibitor and is a useful chemical tool for plant biology and agrochemical studies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656596 | PMC |
| http://dx.doi.org/10.1038/s41598-017-14332-w | DOI Listing |
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