AI Article Synopsis
- This journal expands on previous research regarding how arginine and other solvent additives interact with proteins, specifically in the context of column chromatography.
- The authors discuss their earlier studies on various solvent additives—like sugars, salts, and polymers—and how these interactions lead to important insights into protein stabilization and precipitation effects, linked to the Hofmeister series.
- The rapid advancements in genetic engineering and biotechnology have made this understanding of solvent additives crucial for effectively manipulating protein solutions in practical applications like column chromatography.
Article Abstract
Previously, we have reviewed in this journal (Arakawa, T., Kita, Y., Curr. Protein Pept. Sci., 15, 608-620, 2014) the interaction of arginine with proteins and various applications of this solvent additive in the area of protein formulations and downstream processes. In this special issue, we expand the concept of protein-solvent interaction into the analysis of the effects of solvent additives on various column chromatography, including mixed-mode chromatography. Earlier in our research, we have studied the interactions of such a variety of solvent additives as sugars, salts, amino acids, polymers and organic solvents with a variety of proteins, which resulted in mechanistic understanding on their protein stabilization and precipitation effects, the latter known as Hofmeister series. While such a study was then a pure academic research, rapid development of genetic engineering technologies and resultant biotechnologies made it a valuable knowledge in fully utilizing solvent additives in manipulation of protein solution, including column chromatography.
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