The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4. TSA pretreatment increased DNMT3a, DNMT1, HDAC1, and HAT1 expression, and attenuated them when TAS higher than 50 nM. The protein levels of H3K9ac and H4K8ac in GCs were increased at 48 h after TSA treatment. TSA stimulated the secretion of estradiol and progesterone at a moderate dose. Our data suggest that TSA is important as a regulator of steroid hormone synthesis in granulosa cells during follicular development in the sika deer ovary.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cbin.10899 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Alzheimer's disease (AD) is a neurodegenerative disorder of the central nervous system. The interplay between the intestinal microbiota and metabolites is believed to influence brain function and the pathogenesis of neurodegenerative conditions through the microbe-gut-brain axis. Sika deer antler protein possesses neuroprotective properties; however, the precise mechanism by which it improves AD remains unclear.
View Article and Find Full Text PDFDeer antler reserve mesenchyme cells modified with miR-145 promote chondrogenesis in cartilage regeneration.
Front Vet Sci
December 2024
Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, China.
Deer antler-derived reserve mesenchyme cells (RMCs) are a promising source of cells for cartilage regeneration therapy due to their chondrogenic differentiation potential. However, the regulatory mechanism has not yet been elucidated. In this study, we analyzed the role of microRNAs (miRNAs) in regulating the differentiation of RMCs and in the post-transcriptional regulation of chondrogenesis and hypertrophic differentiation at the molecular and histological levels.
View Article and Find Full Text PDFDetection of host vertebrate DNA in tick species collected from vegetation in Fukuoka, Japan.
Jpn J Infect Dis
December 2024
Division of Pathology and Bacteriology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, Japan.
Ticks are vectors of tick-borne diseases (TBDs) between humans and wild vertebrates. The relationship between ticks, host vertebrates, and their pathogens should be investigated for the effective control of TBDs. Hence, this study aimed to detect vertebrate DNA in ticks by using molecular methods and identify the species of such ticks collected in Fukuoka Prefecture, which is located in the northern Kyushu area of Japan.
View Article and Find Full Text PDFMycobacterium tuberculosis Rv3435c gene regulates inflammatory cytokines and is involved in lung injury and mycobacterial survival in mice.
Microb Pathog
January 2025
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China; Jilin Province Sika Deer Efficient Breeding and Product Development Technology Engineering Research Center, Changchun, China; The Ministry of Education Key Laboratory of Animal Production and the Product Quality and Safety, Changchun, China. Electronic address:
Mycobacterium tuberculosis enters the body through the respiratory tract, produces and releases virulence proteins through a variety of mechanisms, regulates the host immune mechanism through a variety of ways, and then survives in the body for a long time. These depend on virulence genes encoded by Mycobacterium tuberculosis. Previous studies found that the Rv3435c gene of Mycobacterium tuberculosis is highly conserved in pathogenic mycobacterium, but not conserved in non-pathogenic mycobacterium, which may be a potential virulence gene, and inhibit the secretion of inflammatory factors in RAW264.
View Article and Find Full Text PDFComparison of the composition, immunological activity and anti-fatigue effects of different parts in sika deer antler.
Front Pharmacol
December 2024
College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Background: Sika deer (, 1838) antler is a highly esteemed tonic renowned for its abundant assortment of polypeptides, polysaccharides, amino acids, and minerals, and is recognized for its multifarious pharmacological properties. However, limited research has been conducted regarding the variation in composition of deer antlers between the upper and basal sections, as well as their pharmacological effects on immunological activity and anti-fatigue in mice. The objective of this study was to conduct a comprehensive analysis on the appearance, chemical composition, and pharmacological effects of different components within sika deer antlers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!