A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessioni3hnla188fqv2qnpqku3mntuucisslha): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

Cloning and expression of P67 protein of . | LitMetric

Aim: The present study was undertaken to clone, express and study the immunogenicity of P67 protein of .

Materials And Methods: P67 gene was amplified from genomic DNA of . The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent 10G cells. Recombinant protein expression was done by inducing cells with 0.2% Rhamnose. Purification was done using nickel nitrilotriacetic acid affinity chromatography. Western blot and dot blot analysis were performed to assess the immunoreactivity of P67 protein.

Results: PCR amplicon size of P67 gene was found to be 1500 base pair. The size of the fusion protein with SUMO tag was 79 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The recombinant P67 fusion protein expressed in pRham N-His SUMO Kan vector was found to be immunogenic in both western blot and dot blot analysis.

Conclusion: Western blot and dot blot analysis of P67 protein of revealed that the protein is immunogenic. Further work is needed to evaluate the role of P67 antigen of as an immunodiagnostic agent.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639110PMC
http://dx.doi.org/10.14202/vetworld.2017.1108-1113DOI Listing

Publication Analysis

Top Keywords

p67 protein
12
western blot
12
blot dot
12
dot blot
12
p67
8
p67 gene
8
prham n-his
8
n-his sumo
8
sumo kan
8
kan vector
8

Similar Publications

Background: Oxidative stress is a significant cause in the occurrence of cardiac insufficiency. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase2 (NOX2)-derived reactive oxygen species (ROS) play a pivotal role in oxidative stress-induced excitation-contraction decoupling. Stachydrine hydrochloride (Sta) reduces pressure overload-induced cardiac insufficiency, which may be related to the NOX2-ROS pathway, as demonstrated by our earlier research.

View Article and Find Full Text PDF

NPA7: A Dual Receptor Activating Peptide That Inhibits Cardiac Oxidative Stress.

Hypertension

March 2025

Cardiorenal Research Laboratory, Department of Cardiovascular Medicine (Xiaoyu Ma, J.C.M., D.G.M., Xiao Ma, Y.Z., S.P., Y.W., S.J.S., J.C.B.), Mayo Clinic, Rochester, MN.

Background: Cardiomyocyte oxidative stress significantly contributes to the progression of hypertension-induced heart failure, highlighting the need for targeted therapies. We developed a novel peptide, NPA7, that coactivates the GC-A (guanylyl cyclase A)/cGMP and MasR (Mas receptor)/cAMP pathway. This study aimed to test NPA7's ability to inhibit oxidative stress by modulating the p62 (Sequestosome 1)-KEAP1 (Kelch-like ECH-associated protein 1)-NRF2 (nuclear factor erythroid 2-related factor 2) pathway in human cardiomyocytes (HCMs) and a rat model of hypertension.

View Article and Find Full Text PDF

Investigating the link between Helicobacter pylori infection and psoriatic disease: an immunological study.

Immunol Res

December 2024

Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, 41110, Greece.

Helicobacter pylori (Hp) has been postulated as an infectious trigger of psoriatic disease, namely psoriasis (Ps) and psoriatic arthritis (PsA), but meticulous antibody (ab) reactivity against all dominant and subdominant Hp antigens in demographically matched PsA and Ps patients and healthy controls has not been performed so far. IgG anti-Hp ab testing was performed by combining immunoblotting and line assays in 263 serum samples from 89 patients with PsA, 114 patients with Ps, and 60 demographically matched healthy controls (HCs). Anti-Hp positivity did not differ between PsA, Ps, and HCs (P > 0.

View Article and Find Full Text PDF
Article Synopsis
  • The phagocyte NADPH oxidase (NOX2) is essential for the innate immune system, producing reactive oxygen species that help destroy pathogens.
  • Researchers used circular-dichroism analyses alongside past data to assess structural models of the NADPH oxidase complex created by the AI program AlphaFold2.
  • The findings detail how specific interactions and disordered regions within proteins, particularly between p47 and cytb, play a critical role in the assembly and activation of the NADPH oxidase complex.
View Article and Find Full Text PDF

Intact NOX2 in T Cells Mediates Pregnancy-Induced Renal Damage in Dahl SS Rats.

Hypertension

November 2024

Department of Physiology, Medical College of Georgia, Augusta University (J.H.D., J.M.A.-B., S.D.W., E.C.B.-R., M.C.-S., K.E.B., D.L.M.).

Background: Hypertensive disorders of pregnancy are associated with increased risk for cardiovascular disease, renal disease, and mortality. While the exact mechanisms remain unclear, T cells and reactive oxygen species have been implicated in its pathogenesis. We utilized Dahl salt-sensitive (SS), SS (Dahl SS CD247 knockout rat; lacking T cells), and SS (Dahl SS p67 [NOX2 (NADPH [nitcotinamide adenine dinucleotide phosphate] oxidase 2)] knockout rat; lacking NOX2) rats to investigate these mechanisms in primigravida and multigravida states.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!