AI Article Synopsis
- The study presents a new method for creating human intestinal organoids (HIOs) using a defined synthetic hydrogel instead of tumour-derived extracellular matrix, which enhances the potential for regenerative medicine.
- The synthetic hydrogel supports the growth and expansion of HIOs without the risks associated with undefined ECM sources.
- It also proves effective as an injection vehicle for delivering HIOs into damaged intestinal tissue, promoting healing and demonstrating potential therapeutic applications for intestinal injuries.
Article Abstract
In vitro differentiation of human intestinal organoids (HIOs) from pluripotent stem cells is an unparalleled system for creating complex, multicellular three-dimensional structures capable of giving rise to tissue analogous to native human tissue. Current methods for generating HIOs rely on growth in an undefined tumour-derived extracellular matrix (ECM), which severely limits the use of organoid technologies for regenerative and translational medicine. Here, we developed a fully defined, synthetic hydrogel based on a four-armed, maleimide-terminated poly(ethylene glycol) macromer that supports robust and highly reproducible in vitro growth and expansion of HIOs, such that three-dimensional structures are never embedded in tumour-derived ECM. We also demonstrate that the hydrogel serves as an injection vehicle that can be delivered into injured intestinal mucosa resulting in HIO engraftment and improved colonic wound repair. Together, these studies show proof-of-concept that HIOs may be used therapeutically to treat intestinal injury.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664213 | PMC |
| http://dx.doi.org/10.1038/ncb3632 | DOI Listing |
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