Therapeutic biologics have various advantages over synthetic drugs in terms of selectivity, their catalytic nature, and, thus, therapeutic efficacy. These properties offer the potential for more effective treatments that may also overcome the undesirable side effects observed due to off-target toxicities of small molecule drugs. Unfortunately, systemic administration of biologics is challenging due to cellular penetration, renal clearance, and enzymatic degradation difficulties. A delivery vehicle that can overcome these challenges and deliver biologics to specific cellular populations has the potential for significant therapeutic impact. In this work, we describe a redox-responsive nanoparticle platform, which can encapsulate hydrophilic proteins and release them only in the presence of a reducing stimulus. We have formulated these nanoparticles using an inverse emulsion polymerization (IEP) methodology, yielding inverse nanoemulsions, or nanogels. We have demonstrated our ability to overcome the liabilities that contribute to activity loss by delivering a highly challenging cargo, functionally active caspase-3, a cysteine protease susceptible to oxidative and self-proteolytic insults, to the cytosol of HeLa cells by encapsulation inside a redox-responsive nanogel.
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http://dx.doi.org/10.1021/acs.molpharmaceut.7b00643 | DOI Listing |
Langmuir
January 2025
Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India.
Water-in-oil emulsions are critical in various fields, including food, agriculture, personal care, and pharmaceuticals. In some situations, spontaneous emulsification occurs in emulsions with high concentrations of oil-soluble surfactants, in which the parent water drops fragment into finer droplets, forming a network near the interface, which exhibits interfacial elasticity. This study investigates this phenomenon using a water/Span 80-paraffin oil system.
View Article and Find Full Text PDFSoft Matter
January 2025
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
The impact of animal-based food production on climate change drives the development of plant-based alternatives. We demonstrate the use of colloidal thermogelation on a real nanoemulsion system to create structured gels that could be of interest for thermo-mechanical processing of next-generation plant-based food applications. We use a commercial pea protein isolate (PPI) without further purification to stabilize a 20 vol% peanut oil-in-water nanoemulsion at pH = 7 by high-pressure homogenization (HPH) and demonstrate the temperature induced gelation behavior of the nanoemulsion as a function of the HPH processing parameters.
View Article and Find Full Text PDFPhys Chem Chem Phys
January 2025
Laboratorio de Espectroscopía Atómica y Molecular (LEAM), Universidad Industrial de Santander, Colombia.
Illite mineral is present in shale rocks, and its wettability behavior is significant for the oil and gas industry. In this work, the pH effects on the affinity between the (001) and (010) crystallographic planes of illite K(SiAl)(AlMg)O(OH) and direct and inverse emulsions were studied using molecular dynamics simulations. To develop the simulations, an atomistic model of illite was constructed following Löwenstein's rule.
View Article and Find Full Text PDFJ Colloid Interface Sci
March 2025
College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, China; National Manufacturing Innovation Center of Advanced Dyeing and Finishing Technology, Donghua University, Shanghai 201620, China. Electronic address:
Traditional linear polymer is commonly used for polymer flooding in tertiary oil recovery. However, it faces several problems, such as early injection allocation before use and viscosity reduction caused by high-speed shear. In this paper, a novel method of polymer flooding was proposed by using a super absorbent microsphere emulsion.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
March 2025
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, CS 87036, Italy; Macrofarm s.r.l., c/o Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, CS 87036, Italy.
Emerging zoonoses pose significant public health risks and necessitate rapid and effective treatment responses. This study enhances the technology for preparing Molecularly Imprinted Polymers (MIPs), which function as synthetic nanoparticles targeting SARS-CoV-2 receptor-binding domain (RBD), specifically the Omicron variant, thereby inhibiting its function. This study builds on previous findings by introducing precise adjustments in the formulation and process conditions to enhance particle stability and ensure better control over size and distribution, thereby overcoming the issues identified in earlier research.
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