In the present article we consider an accurate modeling of a spectrograph with a cascade of volume-phase holographic gratings. The proposed optical scheme allows us to detect spectra in an extended wavelength range without gaps, providing relatively high spectral resolution and high throughput. However, modeling and minimization of possible cross-talk between gratings and stray light in such a scheme represents a separate task. We use analytical equations of the coupled-wave theory together with rigorous coupled-wave analysis to optimize the gratings parameters and further apply the latter together with a non-sequential ray-tracing algorithm to model propagation of beams through the spectrograph. The results show relatively high throughput up to 53% and the absence of any significant cross-talk or ghost images, even for ordinary holograms recorded on dichromated gelatin.
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http://dx.doi.org/10.1364/AO.56.004284 | DOI Listing |
Adv Sci (Weinh)
January 2025
Tissue Engineering and Organ Manufacturing (TEOM) Lab, Department of Biomedical Engineering, Wuhan University TaiKang Medical School (School of Basic Medical Sciences), Wuhan, 430071, China.
Liver organoids have been increasingly adopted as a critical in vitro model to study liver development and diseases. However, the pre-vascularization of liver organoids without affecting liver parenchymal specification remains a long-lasting challenge, which is essential for their application in regenerative medicine. Here, the large-scale formation of pre-vascularized human hepatobiliary organoids (vhHBOs) is presented without affecting liver epithelial specification via a novel strategy, namely nonparenchymal cell grafting (NCG).
View Article and Find Full Text PDFTrends Biotechnol
January 2025
Department of Food Safety/Hygiene and Risk Management, National Cheng Kung University, Tainan, Taiwan; Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan. Electronic address:
Bacterial proteome microarrays are high-throughput, adaptable tools that allow the simultaneous investigation of thousands of proteins from various bacterial species. These arrays are used to explore bacterial pathogenicity, pathogen-host interactions, and clinical diseases. Recent advancements have expanded their application to profiling human antibodies, identifying biomarkers for infectious and autoimmune diseases, and studying antimicrobial peptides (AMPs).
View Article and Find Full Text PDFBioresour Technol
January 2025
Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, 9000, Belgium; Bio Base Europe Pilot Plant (BBEPP), Rodenhuizekaai 1, Ghent, 9042, Belgium. Electronic address:
Abundant biomass, including industrial waste streams and second-generation (2G) and third-generation (3G) feedstocks, offers significant potential for sustainable bioconversion, nevertheless challenges such as fermentation inhibitors, CO losses and substrate selectivity of traditional microbial hosts hinder process efficiency. In this study, we address these challenges by exploring acetogenic bacteria as alternative microbial hosts. Using a newly established high-throughput method, acetogens were evaluated for their capacity to hydrolyse and metabolise variety of substrates derived from 2G and 3G feedstocks and industrial waste streams.
View Article and Find Full Text PDFBenef Microbes
January 2025
Université Paris-Saclay, 27057INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
Probiotics are widely used for their health promoting effects, though a lot remain to be discovered, particularly on their mechanisms of action at the molecular level. The functional genomic approach is an appropriate method to decipher how probiotics may influence human cell fate and therefore contribute to their health benefit. In the present work, we focused on Shouchella clausii (formerly named Bacillus then Alkalihalobacillus clausii), a spore-forming bacterium that is commercially available as a probiotic for the prevention and the treatment of intestinal dysbiosis and related gastrointestinal disorders, such as diarrhoea.
View Article and Find Full Text PDFJ Pharm Biomed Anal
December 2024
Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. Electronic address:
Qingwen Zhike prescription (QWZK), a traditional Chinese medicine (TCM) hospital prescription developed in response to the corona virus disease 2019 (COVID-19) pandemic, has demonstrated efficacy in clinical practice. Nevertheless, its specific antiviral components and mechanisms of action remain unclear. This study screened the antiviral compounds against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from Qingwen Zhike prescription and explored the underlying mechanism through chemical composition analysis, serum and lung exposure profiles analysis, high-throughput screening, and transmission electron microscopy (TEM) observation.
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