The Early Dendritic Cell Signaling Induced by Virulent Strain Occurs in Phases and Involves the Activation of Extracellular Signal-Regulated Kinases (ERKs) and p38 In the Later Stage.

Dendritic cells (DCs) infected by are poorly activated and do not undergo classical maturation process. Although reasons of such unresponsiveness are not fully understood, their impact on the priming of immunity is well appreciated. Previous attempts to explain the behavior of -infected DCs were hypothesis-driven and focused on events at later stages of infection. Here, we took an alternative unbiased approach by applying methods of global phosphoproteomics to analyze the dynamics of cell signaling in primary DCs during the first hour of infection by Presented results show that the early response of DCs to occurs in phases and that ERK and p38 signaling modules induced at the later stage are differentially regulated by virulent and attenuated Δ strain. These findings imply that the temporal orchestration of host proinflammatory pathways represents the integral part of life-cycle inside hijacked DCs.