AI Article Synopsis
- Repetitive DNA sequences, especially trinucleotide repeats, can lead to various hereditary disorders in humans due to their instability.
- This study presents a new method to analyze the instability of these triplet repeats using yeast as a model organism.
- The researchers utilize a fluctuation assay combined with a software called FluCalc to measure the rates of repeat expansions and associated genetic mutations.
Article Abstract
Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741187 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-7306-4_29 | DOI Listing |
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