AI Article Synopsis
- Ribonucleotides in DNA make it prone to single-stranded breaks when exposed to alkali, highlighting their importance in genomic stability.
- Hydrolytic end sequencing (HydEn-seq) is introduced as a next-gen sequencing technique to locate these ribonucleotides in yeast lacking ribonucleotide excision repair.
- This method is applied to explore various genomic characteristics in both wild-type and specific variant yeast strains.
Article Abstract
Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.
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| http://dx.doi.org/10.1007/978-1-4939-7306-4_23 | DOI Listing |
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