A rapid and efficient DNA extraction method suitable for marine macroalgae.

3 Biotech

Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu 603203 India.

Published: December 2017

Macroalgae are a diverse group of organisms. Marine macroalgae, in particular, have numerous medicinal and industrial applications. Molecular studies of macroalgae require suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from macroalgae. However, they are either time consuming, expensive or work only with few species. The method described in this study is rapid and efficient and applicable to different types of marine macroalgae. This method yields an average of 3.85 µg of DNA per 50 mg of algal tissue, with an average purity of 1.88. The isolated DNA was suitable for PCR amplification of universal plastid region of macroalgae.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628057PMC
http://dx.doi.org/10.1007/s13205-017-0992-2DOI Listing

Publication Analysis

Top Keywords

marine macroalgae
12
rapid efficient
8
dna extraction
8
macroalgae
7
dna
5
efficient dna
4
extraction method
4
method suitable
4
suitable marine
4
macroalgae macroalgae
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!