We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4% for the index of refraction and 2.2% for the diameter of the particles.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OE.25.025853 | DOI Listing |
Publication Analysis
Top Keywords
holography microscopy
8
microscopy based
8
based lorenz-mie
8
low coherence
4
coherence digital
4
digital holography
4
lorenz-mie scattering
4
model
4
scattering model
4
model demonstrate
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!