In mammals, there are four Argonaute (Ago) family proteins that play crucial roles in RNA silencing, a process wherein microRNA (miRNA) mediates inhibition of target mRNA translation. Among the Ago proteins, Argonaute2 (Ago2) uniquely possesses an endoribonuclease (slicer) activity that is critical for the biogenesis of specific miRNAs and mRNA cleavage. This Ago2 slicer activity is required for postnatal development. Despite its important roles, there are still gaps in our understanding of the mechanistic basis of Ago2's unique functions in vivo due to a limited availability of experimental tools. In order to investigate Ago2's functions, we generated a new cellular model of Ago2-deficiency in 3T3 mouse embryonic fibroblasts (MEFs). This cell line can be used for investigating general Ago2 functions, but also for further understanding of Ago2's unique characteristics including the slicer activity, specific amino acid residues, and domains in Ago2 by reconstitution of Ago2 mutants. Here, we describe the methods for establishing Ago2-deficient MEFs and for reconstituting the MEFs with an Ago2 mutant lacking its slicer activity by means of a retrovirus-mediated gene transfer.
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http://dx.doi.org/10.1007/978-1-4939-7339-2_14 | DOI Listing |
BMC Med Imaging
December 2024
Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Introduction: Gadolinium-based T1-weighted MRI sequence is the gold standard for the detection of active multiple sclerosis (MS) lesions. The performance of machine learning (ML) and deep learning (DL) models in the classification of active and non-active MS lesions from the T2-weighted MRI images has been investigated in this study.
Methods: 107 Features of 75 active and 100 non-active MS lesions were extracted by using SegmentEditor and Radiomics modules of 3D slicer software.
Orthod Craniofac Res
October 2024
Department of Orthodontics, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
Objectives: This study aimed to assess the efficacy of active presurgical orthopaedics appliances in infants presenting with complete unilateral cleft lip and overlapped alveolus (T-shaped alveolus).
Materials And Methods: A retrospective and prospective longitudinal study was performed. Infants diagnosed with complete unilateral cleft lip and overlapped alveolus, received treatment with the active presurgical orthopaedics appliances from June 2020 to June 2023, at Children's Hospital 1, Ho Chi Minh City.
J Craniofac Surg
September 2024
Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing Ophthalmology and Visual Science Key Lab, Capital Medical University, Beijing, China.
ACS Biomater Sci Eng
October 2024
Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.
Embedded 3D printing (EMB3D) is an additive manufacturing technique that enables complex fabrication of soft materials including tissues and silicones. In EMB3D, a nozzle writes continuous filaments into a support bath consisting of a yield stress fluid. Lack of fusion defects between filaments can occur because the nozzle pushes support fluid into existing filaments, preventing coalescence.
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August 2024
Anatomy, All India Institute of Medical Sciences, Bathinda, Bathinda, IND.
Background: Underlying disorders of the maxillary sinus (MS), including a history of sinus surgeries, chronic sinusitis, or congenital anomalies can potentially impact sinus function and structure, necessitating careful evaluation and management. Moreover, intact sinuses are crucial in gender determination in forensic anthropology. The present study was undertaken to check the accuracy and reliability of MS in gender determination using morphometric parameters.
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